Oroku Saki
Defeat Saki as Raphael, Donatello, Michaelangelo, or Leonardo to unlock Oroku Saki in versus mode.
Casey Jones
Successfully complete level 1 as Raphael to unlock Casey Jones in versus mode.
Evil Turtlebot
Successfully complete level 3 as Raphael, Donatello, Michaelangelo, or Leonardo to unlock the Evil Turtlebot in versus mode.
Hun
Successfully complete level 6 as Michaelangelo to unlock Hun in versus mode.
Challenge mode
Successfully complete story mode as Raphael, Donatello, Michaelangelo, and Leonardo.
Shredder
Defeat Shredder as Raphael, Donatello, Michaelangelo, or Leonardo to unlock Shredder in versus mode.
Splinter
Successfully complete Dojo level 3 as Leonardo to unlock Splinter in versus mode.
Yoshi Hamato
Successfully complete Challenge Mode as Raphael, Donatello, Michaelangelo, or Leonardo to unlock Yoshi Hamato in versus mode.
Unleash the Secret of Gembu
Beat the third Dojo Stage with all four turtles to learn the Secret of Gembu maneuever. Use the move to damage everyone on the screen. You'll have three opportunities per level to use it.
Alternate costumes
Set the system date to December 25 to see the turtles with a Christmas theme. Set the system date to October 31 to see the turtles with a Halloween theme.
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